NOTES ON THE CULTIVATION OF TREPONEMA PALLIDUM
نویسندگان
چکیده
منابع مشابه
The Direct Cultivation of Treponema Pallidum Pathogenic for the Monkey
A method for the direct cultivation of Treponema pallidum from human syphilitic lesions, by the employment of a solid medium, has been described. By means of it, three of the four strains worked with were successfully cultivated. The several pure cultures agree in morphological and cultural characters, grow only in the presence of sterile tissue under anaerobic conditions, and do not produce pu...
متن کاملShape of Treponema pallidum.
Treponema pallidum was found to be not helical, but a flat wave twisted into one to five different planes per cell.
متن کاملMucopolysaccharidase of Treponema pallidum.
Treponema pallidum (Nichols strain) exhibited mucopolysaccharidase activity. Acidic mucopolysaccharides were broken down more rapidly by viable treponemes than by heat-inactivated treponemes or membrane filtrates of treponemal suspensions. Ouchterlony immunodiffusion demonstrated the occurrence of antibodies to the hyaluronidase-like enzyme within syphilitic sera. After intratesticular inoculat...
متن کاملCultivation of cottontail rabbit epidermal (Sf1Ep) cells on microcarrier beads and their use for suspension cultivation of Treponema pallidum subsp. pallidum.
In vitro propagation of Treponema pallidum can be achieved by cocultivation with Sf1Ep cells. This study had two objectives: (i) to achieve suspension cultivation of Sf1Ep cells and (ii) to develop procedures for achieving the replication of T. pallidum in those cell cultures. Seven suspension cultures of Sf1Ep cells yielded an average of 7.2 x 10(8) T. pallidum (36-fold increase) after 12 days.
متن کاملStudies on Treponema Pallidum and Syphilis. Ii. Spiroch~ticidal Antibodies against Treponema Pallidum. by Hans
In a previous communication we have reported on the agglufinins which are formed in rabbits following systematic inoculation with concentrated culture material of Treponema pallidum. Incidentally it may be stated that a sheep has also been successfully used. The antigen used for the immunization of these animals was similar to that employed in the first agglutination experiments, except that we...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1915
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.21.3.213